Suppression of Metalloproteinase Biosynthesis

To study the interaction of lymphocytes and macrophages in the control of extracellular matrix turnover, we determined the effects ofseveral solubleT cell products on mononuclear phagocyte production of metalloproteinases. Cytokines including IL2, IL4, IL-6, tumor necrosis factor a (TNFa), GM-CSF, and IFN-gamma were each tested for capacity to modulate macrophage metalloproteinase and tissue inhibitor of metalloproteinases (TIMP) expression. The addition ofIL4 to cells cultured under basal conditions caused a dose-dependent suppression in the release of 92-kD type IV collagenase without affecting TIMP production. 92-kD enzyme secretion was inhibited by 50% with 1-2 ng/ ml ofIL4 and by 90% with 10 ng/ml ofIL4. When cells were first exposed to killed Staphylococcus aureus to induce metalloproteinase production, IL4 potently blocked the stimulated release of both interstitial collagenase and 92kD type IV collagenase, again without effect uponTIMP. Metabolic labeling experiments and Northern hybridizations demonstrated that IL4 exerted its action at a pretranslational level. Furthermore, IL4 possessed the capacity to inhibit metalloproteinase expression even in the relatively immature peripheral blood monocyte. As reported previously (Shapiro, S. D., E. J. Campbell, D. K. Kobayashi, and H. G. Welgus. 1990. J. Clin. Invest. 86:1204), IFN-gamma suppressed constitutive macrophage production of 92-kD type IV collagenase. Despite the frequent antagonism observed between IL-4 and IFNgamma in other systems, the combination of these two agents lowered metalloproteinase biosynthesis dramatically, whereas IL-4 opposed the IFN-gamma-stimulated production of cytokines (IL-1 and TNFa). IL-6 had only minimal effect upon metalloproteinase production, but appeared to specifically augment TIMP release. In summary, cytokines released by activated T cells may profoundly reduce the capacity of the macrophage to mediate extracellular matrix degradation. (J. Clin. Invest. 1992. 90:382-388.)